diff --git a/contracts/contract/megapool/RocketMegapoolManager.sol b/contracts/contract/megapool/RocketMegapoolManager.sol
index 9e2ca689..73bc0bf2 100644
--- a/contracts/contract/megapool/RocketMegapoolManager.sol
+++ b/contracts/contract/megapool/RocketMegapoolManager.sol
@@ -40,8 +40,8 @@ contract RocketMegapoolManager is RocketBase, RocketMegapoolManagerInterface {
         setUint(setCountKey, index + 1);
         uint256 encoded = (uint256(uint160(_megapoolAddress)) << 96) | _validatorId;
         setUint(keccak256(abi.encodePacked("megapool.validator.set", index)), encoded);
-        // Add pubkey => megapool mapping and ensure uniqueness
-        bytes32 key = keccak256(abi.encodePacked("validator.megapool", _megapoolAddress, _pubkey));
+        // Add pubkey => megapool mapping and ensure uniqueness across all megapools
+        bytes32 key = keccak256(abi.encodePacked("validator.megapool", _pubkey));
         require(getAddress(key) == address(0x0), "Pubkey in use");
         setAddress(key, _megapoolAddress);
     }
diff --git a/test/megapool/megapool-tests.js b/test/megapool/megapool-tests.js
index ef2f0e0e..c5115f95 100644
--- a/test/megapool/megapool-tests.js
+++ b/test/megapool/megapool-tests.js
@@ -409,6 +409,38 @@ export default function() {
             );
         });
 
+        it(printTitle('node', 'can not reuse pubkey across megapools'), async () => {
+            const rocketNodeDeposit = await RocketNodeDeposit.deployed();
+            const pubkey = getValidatorPubkey();
+            const signature = getValidatorSignature();
+
+            const withdrawalCredentials1 = await getMegapoolWithdrawalCredentials(node.address);
+            const depositData1 = {
+                pubkey,
+                withdrawalCredentials: Buffer.from(withdrawalCredentials1.substr(2), 'hex'),
+                amount: BigInt(1000000000), // gwei
+                signature,
+            };
+            const depositDataRoot1 = getDepositDataRoot(depositData1);
+
+            await rocketNodeDeposit.connect(node).deposit('4'.ether, false, pubkey, signature, depositDataRoot1, { value: '4'.ether });
+
+            const withdrawalCredentials2 = await getMegapoolWithdrawalCredentials(node2.address);
+            const depositData2 = {
+                pubkey,
+                withdrawalCredentials: Buffer.from(withdrawalCredentials2.substr(2), 'hex'),
+                amount: BigInt(1000000000), // gwei
+                signature,
+            };
+            const depositDataRoot2 = getDepositDataRoot(depositData2);
+
+            await shouldRevert(
+                rocketNodeDeposit.connect(node2).deposit('4'.ether, false, pubkey, signature, depositDataRoot2, { value: '4'.ether }),
+                'Was able to reuse existing pubkey across megapools',
+                'Pubkey in use',
+            );
+        });
+
         it(printTitle('node', 'can deposit using ETH credit'), async () => {
             // Enter and exit queue to receive a 4 ETH credit
             await nodeDeposit(node, '4'.ether);